Table 5.
Conserved sequence motifs in JHE and putative JHE sequences from various insect orders
| Order | Enzyme | Signal peptidea |
Motifb |
Reference | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RF | DQ | GQSAG | E | GxxHxxD | R/Kx(6)R/KxxxR | T | ||||
| Lepidoptera | HvJHE | 17 | + | + | + | + | + | Rx(6)RxxxR | + | 40) |
| CfJHE | 21 | + | DM | + | + | + | Rx(6)RxxxR | + | 79) | |
| MsJHE | 22 | + | DM | + | + | + | Rx(6)KxxxR | + | 39) | |
| BmJHE | 19 | + | DM | + | + | + | Rx(6)RxxxR | + | 80) | |
| Coleoptera | TmJHE | 22 | + | + | + | + | + | Kx(6)KxxxE | + | 76) |
| PhJHE | 21 | + | + | + | + | + | Kx(6)KxxxS | N | 81) | |
| TcJHE | 23 | + | + | + | + | + | Kx(6)KxxxE | + | 50) | |
| Diptera | DmJHE | 20 | + | + | + | + | + | Kx(6)RxxxR | + | 51) |
| AaJHE | 23 | + | + | + | D | GxxHxxE | Kx(6)KxxxR | + | 42) | |
| Orthoptera | GaJHE | n.a.b | + | + | + | + | + | Kx(6)RxxxR | + | 46) |
| Hemiptera | NlJHE | 20 | RY | + | + | + | + | Lx(6)KxxxR | H | 78) |
| Hymenoptera | AmJHE | 18 | + | + | GLSAG | + | + | Kx(6)RxxxE | + | 43) |
a
Length of predicted secretion signal peptide. Some secretion signal peptide sequences were predicted using the Signal IP 3.0 server.95)
b
The complete N-terminal sequence is not available (n.a.).
c
The “+” indicates that the consensus sequence is maintained.