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Expression of non-muscle MYH9 and MYH10 by cells in tendon constructs. (A) PCR gel electrophoresis analysis of MYH9 and MYH10 and col1a1 gene expression. (B) Western blot for NMMHC-IIB protein. (C, D, E) Real-time PCR expression of MYH9 (C), MYH10 (D) and col1a1 (E) in tendon constructs between T0 and T21. Expression of MYH9 and MYH10 was significantly reduced in tendon constructs compared to monolayer, whereas col1a1 was significantly increased. ⁎p < 0.05.
