Fig. 11.

Continuous labeling with ¹⁴C-proline. (A) Tendon constructs (at T0 or at T21) were treated with either blebbistatin or 0.1% DMSO for 4 h and then ¹⁴C-proline was added for a further 1 h. Proteins were extracted as previously described (Canty et al., 2004). (B) Sequential salt extractions (S1, S4) for extracellular proteins followed by an NP-40 detergent extraction (N) for intracellular proteins demonstrated that blebbistatin did not affect the ability of the cells to secrete procollagen and to convert procollagen to collagen.