Fig. 12.

Live-cell imaging of cells in tendon constructs. Cells seeded in fibrin gels at T6 were treated with either DMSO, blebbistatin, or cytochalasin and imaged for 12 h at 5-min intervals. (A) Schematic showing the fibrin gel (shaded grey) contracting around the fixed-position pins. The box indicates the area imaged. (B) Cell contraction was highlighted with colored lines tracking the position of individual cells over time. Cell contraction was abolished by treatment with blebbistatin or with cytochalasin. Scale bar, 100 μm.