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. 2012 Aug 2;104(16):1228–1239. doi: 10.1093/jnci/djs321

Figure 3.

Figure 3.

Effects of phenethyl isothiocyanate (PEITC) administration on human epidermal growth factor receptor-2 (HER-2) expression, Ki-67 expression, terminal deoxynucleotidyl transferase–mediated dUTP nick-labeling (TUNEL)–positive bodies, and blood vessels in the tumors of mouse mammary tumor virus–neu mice. A) HER-2 expression in tumors of representative mice placed on the control diet or the PEITC-supplemented diet. Immunohistochemical staining was performed with anti-HER2 antibody (×200 magnification, scale bars = 100 µm). Results represent mean HER-2–positive cells per high power field (n = 6 mice per group, P = .35 by two-sided Student’s t test) with their 95% confidence intervals (error bars). B) Cell proliferation in tumors of representative mice placed on the control diet or the PEITC-supplemented diet (×200 magnification, scale bars = 100 µm). Results represent mean Ki-67–positive cells per high power field (n = 16 for mice placed on the control diet and n = 11 for mice placed on the PEITC-supplemented diet, P = .003 by two-sided Student’s t test) with their 95% confidence intervals (error bars). C) TUNEL-positive apoptotic bodies in tumors of representative mice placed on the control diet or the PEITC-supplemented diet. Apoptotic bodies were visualized by a commercial TUNEL-staining kit (×200 magnification, scale bars = 100 µm). Results represent mean TUNEL-positive bodies per high power field (n = 16 for mice placed on the control diet and n = 10 for mice placed on the PEITC-supplemented diet, P < .001 by two-sided Student’s t test) with their 95% confidence intervals (error bars). D) Blood vessels in tumors of representative mice placed on the control diet or the PEITC-supplemented diet. Immunohistochemistry was performed with anti-CD31 antibody (×200 magnification, scale bars = 100 µm). Results represent mean number of blood vessels per high power field (n = 16 for mice placed on the control diet and n = 11 for mice placed on the PEITC-supplemented diet, P = .05 by two-sided Student’s t test) with their 95% confidence intervals (error bars).