Figure 6.

Effect of phenethyl isothiocyanate (PEITC) administration on expression of WD-repeat containing protein-1 (WDR1), transferrin, ATP synthase, and annexin A4 in the tumors of mouse mammary tumor virus–neu mice. A) Expression of WDR1, a protein associated with actin organization, in tumors of representative mice placed on control diet or PEITC-supplemented diet (×200 magnification, scale bars = 100 µm). Results shown are mean expression (arbitrary units) of WDR1 (n = 7 for mice placed on the control diet and n = 6 for mice placed on the PEITC-supplemented diet) with corresponding 95% confidence intervals (error bars). B) Immunohistochemical images depicting transferrin expression in tumors of representative mice placed on control diet or PEITC-supplemented diet (×200 magnification, scale bars = 100 µm). Results shown are mean expression of transferrin (n = 7 for mice placed on the control diet and n = 6 for mice placed on the PEITC-supplemented diet) with corresponding 95% confidence intervals (error bars). C) Immunohistochemical images depicting ATP synthase expression in tumors of representative mice placed on the control diet or the PEITC-supplemented diet (×200 magnification, scale bars = 100 µm). Results shown are mean expression of ATP synthase (n = 7 for mice placed on the control diet and n = 6 for mice placed on the PEITC-supplemented diet) with corresponding 95% confidence intervals (error bars). D) Immunohistochemical images depicting annexin A4 expression in tumors of representative mice placed on the control diet or the PEITC-supplemented diet (×200 magnification, scale bars = 100 µm). Results shown are mean membrane intensity of annexin A4 expression (n = 5 for mice placed on the control diet and n = 4 for mice placed on the PEITC-supplemented diet) with corresponding 95% confidence intervals (error bars). Statistical significance of differences in panels A to D was determined by two-sided Student’s t tests.