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. Author manuscript; available in PMC: 2013 Mar 29.
Published in final edited form as: Methods Enzymol. 2010 Mar 1;470:797–831. doi: 10.1016/S0076-6879(10)70033-1

Figure 33.3.

Figure 33.3

(A) Example PDS-1000/He biolistic particle delivery system (Bio-Rad): (a) “VENT/HOLD/VAC” toggle switch, (b) “FIRE” toggle switch, (c) gas acceleration tube/retaining cap, (d) microcarrier launch assembly, and (e) plate holder. (B) Close-up of microcarrier launch assembly for biolistic particle delivery system: (d) microcarrier launch assembly, (f) Top to microcarrier launch assembly, (g) Macrocarrier holder. (C) Example of a YPAD plate immediately following biolistic transformation. Note the scattering of microcarriers in the center of the patch of C. neoformans cells.