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. Author manuscript; available in PMC: 2013 Mar 29.
Published in final edited form as: Methods Enzymol. 2010 Mar 1;470:797–831. doi: 10.1016/S0076-6879(10)70033-1

Figure 33.6.

Figure 33.6

(A) Melanin assay: The kinetics of melanization varies depending on the incubation temperature. lac1Δ is deficient in the primary laccase enzyme responsible for melanization in C. neoformans. (B) Capsule formation assay: WT (H99) cell grown under capsule inducting conditions (DMEM, 37 °C, 5% CO2) and visualized with India ink. Bar denotes 10 μm.