Skip to main content
. Author manuscript; available in PMC: 2013 Mar 29.
Published in final edited form as: Methods Enzymol. 2010 Mar 1;470:797–831. doi: 10.1016/S0076-6879(10)70033-1
Composition
YPAD (yeast peptone adenine dextrose)
1% bacto yeast extract (Becton Dickinson, Cat. No.
 212720)		 10 g
2% bacto peptone (Beckton Dickinson, Cat. No.
 211820)		 20 g
2% glucose 20 g
0.73 mM L-tryptophan (Sigma, Cat. No. T8941) 0.15 g
0.27 mM adenine (Sigma, Cat. No. A2786) 0.037 g
Water to 1 l
YNB (yeast nitrogen base)
0.15% YNB w/o amino acids, w/o dextrose, w/o
 ammonium sulfate (BIO 101, Cat. No. 4027-032)		 3 g
75 mM ammonium sulfate 10 g
2% glucose 20 g
Water to 1 l
Sabouraud dextrose
3% Sabouraud dextrose broth (Becton Dickinson,
 Cat. No. 238210)		 30 g
Water to 1 l