| Regeneration medium | Composition |
|---|---|
| 0.9% YNB w/o ammonium sulfate w/o dextrose w/o ammonium sulfate (BIO 101, Cat. No. 4027-032) |
9 g |
| 1 M sorbitol | 182 g |
| 1 M mannitol | 182 g |
| 2.6% glucose | 26 g |
| 0.267% bacto yeast extract (Becton Dickinson, Cat. No. 212720) |
0.27 g |
| 0.054% bacto peptone (Beckton Dickinson, Cat. No. 211820) |
0.54 g |
| 0.133% Gelatin (Sigma, Cat. No. G-8150) | 1.33 g |
| Water | to 1 l |
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