FIGURE 2:

Magnesium is efficient in live but not in fixed HAVSMC. HAVSMC were cultured for 14 days at the indicated concentrations of Mg²⁺ and in the presence of 3 mM Pi. (A) Calcium deposition from Pi-induced HAVSMC of five different donors was assessed using OCP with increasing concentration of Mg²⁺. Data are quoted as a ratio of Pi and represent the mean ± SD of each condition (n = 16). Ct was significantly different Pi. ^#P < 0.05 versus Pi and ^####P < 0.0001 at Day 10. ****P < 0.0001 versus Pi at Day 14. (B) Calcium deposition on fixed HAVSMC of three different donors was assessed using OCP with increasing concentration of Mg²⁺ in the presence of Pi. Data are quoted as a ratio of Pi and represent the mean ± SD of each condition (n = 12). Ct was significantly different from Pi, ****P < 0.0001 versus Pi. No significant differences were found between Ct and Mg²⁺ conditions. No significant differences were found between Pi and Pi + Mg²⁺ conditions. (C) Von Kossa followed by a haematoxylin/Mayer counterstaining was performed on the cell layer of fixed cells (×25 magnification).