Figure 4.

Curcumin attenuated hydrogen peroxide-induced microRNA expression in ARPE-19 cells. In the polymerase chain reaction (PCR) array, cells were treated with 20 μM curcumin for 6 h and 200 μM hydrogen peroxide (H₂O₂) for 18 h. In the curcumin pretreatment group, the cells were treated with 20 μM curcumin for 6 h first, and after washing, the cells were insulted with 200 μM H₂O₂ for 18 h. The curcumin pretreatment significantly suppressed the H₂O₂-induced expression of 17 microRNAs (miRNAs) compared to controls. Data represent mean±SEM from three separate samples. *p<0.05 versus control, **p<0.001 versus control, #p<0.001 versus H₂O₂, FC≥ or ≤2.