Table 1. Primers used for quantitative real-time PCR.
| Gene | Primer sequence (5’-3’) | Amplicon size (bp) |
|---|---|---|
|
VEGF-A |
F: TGCCATCCAATCGAGACCCTG |
156 |
| |
R:GGTGATGTTGGACTCCTCAGTG |
|
|
NF-κB1 |
F:CAACCACAGATGGCACTGCC |
125 |
| |
R:GCACCAGGTAGTCCACCATG |
|
|
AT1R |
F:TGCAGATATTGTGGACACGGCC |
154 |
| |
R:GTGGGATTTGGCTTTTGGGGG |
|
|
Catalase |
F:CCATTATAAGACTGACCAGGGC |
133 |
| |
R:AGTCCAGGAGGGGTACTTTCC |
|
|
GPx-1 |
F:AGTCGGTGTATGCCTTCTCGG |
142 |
| |
R:TCGTTCATCTGGGTGTAGTCCC |
|
|
GPx-4 |
F:GAGTTTTCCGCCAAGGACATCGA |
130 |
| |
R:GGTCGACGAGCTGAGTGTAGTTT |
|
|
Hprt |
F:ACAGGACTGAACGTCTTGCTCG |
87 |
| R:TATAGCCCCCCTTGAGCACAC |
FR, fold regulation; FC, fold change. (Note: FC values greater than one indicate a positive or an upregulation, and the FR is equal to the FC. FC values less than one indicate a negative or downregulation, and the FR is the negative inverse of the FC).