Skip to main content
. 2013 Mar 20;19:604–613.

Figure 5.

Figure 5

Importin 13 regulated keratin 17 in the pterygium epithelial cells. A–E demonstrate knockdown of the IPO13 gene inhibited keratin 17 (K17) expression. A demonstrates quantitative real-time PCR analysis of IPO13 in the control (siRNA-CTR) and IPO13-silenced531 pterygium epithelial cells (PECs; siRNA-IPO13). B demonstrates Western blotting results of PECs, transfected with control and siRNA-IPO13. C: Quantitative real-time PCR analysis of K17 mRNA transcripts in the control and siRNA-IPO13. D demonstrates western blotting results of PECs transfected with control and siRNA-IPO13. E demonstrates representative images of immunofluorescence staining of K17 with anti-K17 antibody in the PECs transfected with control and siRNA-IPO13. DAPI (blue) counterstaining. Silencing of the IPO13 gene significantly inhibited K17 expression. F and G demonstrate overexpression of the IPO13 gene increased K17 expression. F demonstrates representative images of western blotting of PECs transfected with pLV-flag empty vector (control) and IPO13 overexpressing (pLV-flag-IPO13). G demonstrates representative images of immunofluorescence staining of K17 (red) with anti-K17 antibody in the PECs transfected with control and pLV-flag-IPO13. DAPI (blue) counterstaining. Overexpression of the IPO13 gene enhanced K17 expression. Scale bar: 10 μm.