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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Biochemistry. 2012 Aug 22;51(35):6920–6931. doi: 10.1021/bi3007466

Figure 2.

Figure 2

Optimum conditions for Tsr proteoliposome formation. (A & B) The conversion of phospholipid liposomes (2 mg lipid/mL) into mixed micelles was monitored by light scattering at 504 nm as a function of the OG (0.0 to 1.0% w/v) added to the liposomes. OG-solubilized affinity-purified Tsr was added to the solutions and the OG was removed (as described in Materials and Methods) to yield proteoliposomes. (C) The kinase activity of receptor/CheW/CheA complexes was measured in the coupled ATPase assay using Tsr proteolipsomes prepared with different OG concentrations shown in Fig. 2B. Proteoliposomes prepared with either 0.7 or 0.8% OG (w/v) yielded the largest CheA activity