Skip to main content
. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Biochemistry. 2012 Aug 22;51(35):6920–6931. doi: 10.1021/bi3007466

Figure 4.

Figure 4

Kinase activity of receptor/CheW/CheA complexes as a function of the lipid to protein ratio in proteoliposomes. Receptor/CheW/CheA complex assembly conditions: 16 μM receptor, 7 μM CheA and 10 μM CheW incubated overnight at 25°C. Kinase activities (32P incorporation assay) are scaled to the total amount of CheA added (7 μM, left y-axis) and to the amount of CheA bound in the complex (right y-axis). (A) Kinase activities of Tsr4E proteoliposomes without serine (white triangles) and with 10 mM serine (black triangles). (B) Kinase activities of Tsr4Q proteoliposomes without serine (white diamonds) and with 10 mM serine (black diamonds). In contrast to the locked-on property of Tsr4Q proteoliposomes, a 100:1 4E sample included in the same series is inhibited 90% by 10 mM serine (black triangle).