Fig. 2.
PKA and CAMKII do not change LysoTracker fluorescence in retinal bipolar terminals. (A) Panel showing representative cell terminals stained with LT 100 nM (first panel) in association with NH4Cl 30 mM (second panel); SP-cAMP 100 nM (third panel), RP-cAMP 100 nM (fourth panel), and KN93 1 μM (fifth panel). Scale bar = 10 μM. Upper row, DIC images; lower row, fluorescence images. (B) Graphic showing that cells exposed to NH4Cl were less stained with LT compared to control (*P < 0.05; n = 8) whereas SP-cAMP (P > 0.05; n = 10), RP-cAMP (P > 0.05; n = 9) and KN93 (P > 0.05; n = 9) did not cause significant changes in fluorescence in retinal bipolar terminals if compared to their controls.
