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. 2013 Mar 29;8(3):e60569. doi: 10.1371/journal.pone.0060569

Figure 4. qRT-PCR Analyses of Genes Involved in Osteoclastogenesis and Osteoblast Activity.

Figure 4

Bone marrow (A) was analyzed for relative mRNA abundance of the osteoclastgensis gene nuclear factor of activated T-cells (Nfatc1), while the flushed femur (B-D) was used to assess genes involved in osteoblast activity and function: alkaline phosphatase (Alp), type 1 collagen (Col1a1), and osteocalcin (Ocn) in sham-operated (Sham) and ovariectomized (OVX) mice fed control diet, or control diet supplemented with either 25% (w/w) dried plum, apple, apricot, grape, or mango. Bars represent the mean ± SE, n  =  6 mice in each group. Bars that share the same superscript letter are not significantly different from each other (p<0.05).