Figure 1. The effects of AnxA2, S100A10 and the heterotetrameric AnxA2₂-S100A10₂ complex on the formation of an in vitro capillary-like network.

Co-cultures of SMCs and GFP-expressing HUVECs were untreated (A), or treated with 15 µM AnxA2 (B), 15 µM S100A10 (C), 6 µM AnxA2₂-S100A10₂ complex (12 µM AnxA2) (D) or 100 nM PTK787 (E) at 2 h after seeding. After 72 h incubation, images were taken at 10× magnification. The tube total length was analysed (F) and expressed as percentage relative to the untreated EC control (100%) (A) using the Attovision and BD Image Data Explorer programmes. Results (F) are the mean ± SEM of 3 independent experiments each. Statistical significance was determined by the two-tailed Student's t-test (*P<0.05).