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. 2013 Mar 29;8(3):e60281. doi: 10.1371/journal.pone.0060281

Figure 3. The effect of monoclonal AnxA2 antibodies on the formation of an in vitro capillary-like network.

Figure 3

Co-cultures of SMCs and GFP-expressing HUVECs were untreated (A), or treated with 4 µg/200 µl monoclonal AnxA2 antibodies (BD Biosciences) (B), (C-10; Santa Cruz), (D), Tyr23 AnxA2 specific antibodies (Santa Cruz) (F) or normal mouse IgG (H) at 2 h after seeding. After 72 h incubation, images were taken at 10× magnification. The tube total length was analysed (C, E, G and I) and expressed as percentage relative to the corresponding untreated EC control (100%) (A), as described for Figure 1. Results (C, E, G and I) are the mean ± SEM of 3 independent experiments each. Statistical significance was determined by the two-tailed Student's t-test (*P<0.05).