Skip to main content
. 2013 Mar 29;8(3):e60281. doi: 10.1371/journal.pone.0060281

Figure 4. Tyr23 phosphorylated AnxA2 is present in the ECM of confluent HUVECs.

Figure 4

HUVECs were incubated for 30 min in the absence (control) or presence of 50 µM PP2 before harvesting. The control fraction (ECM) was obtained in the presence of 200 µM ortho-vanadate to inhibit dephosphorylation. 100 µg of EGTA-released extracellular proteins were subjected to 10% SDS-PAGE and subsequently transferred to a nitrocellulose membrane for the detection of AnxA2 by Western blot analysis using monoclonal antibodies directed against pTyr23 AnxA2 (A) or AnxA2 (BD Biosciences) (B). Selected standards are indicated by arrowheads to the left. AnxA2 and actin (as a loading control) are indicated by an arrowhead and asterisk, respectively.