Figure 5. The effects of AnxA2, S100A10, AnxA2-D_I, or AnxA2-D_IV on in vitro preformed capillary-like networks.

Co-cultures with a preformed EC network were treated with 15 µM AnxA2 (B), S100A10 (C), AnxA2-D_I (D), or AnxA2-D_IV (E). After 72 h incubation, images were taken at 10× magnification. The degree of disruption of the mature vascular network was quantified (F) as described for Figure 1. The tube total length is expressed as percentage relative to the untreated EC control (100%) (A). Results (F) are the mean ± SEM of 3 independent experiments each. Statistical significance was determined by the two-tailed Student's t-test (*P<0.05).