Strains
E. coli
|
|
|
Ec002 |
RifampicinR derivative of DH5α: F- endA hsdR17 supE44 thi-1 λ- recA1 gyrA96 relA1 φ80 dLacZΔM15 |
This study |
Ec003 |
Ec002 carrying pJEG011 and pJEG012 |
This study |
K. pneumoniae Kp001 |
Clinical outbreak-associated isolate carrying pJEG011 and pJEG012 |
This study |
Kp002 |
Clinical outbreak-associated isolate phenotypically and genotypically indistinguishable from Kp001: isolated after 18 months of intra-abdominal carriage |
This study |
Plasmids
|
|
|
pJEG011 |
IncL/M, Tn1999, ISEcp1-bla
CTX-M-14, Tn5393jΔ |
This study |
pJEG012 |
pir, Tn1331
|
This study |
Primers
|
|
|
aacA4-F |
5′- gaagagtatgagtattcaaacatttcc -3′ |
This study |
aacA4-R |
5′- ggaagggttaggcaacac -3′ |
This study |
aacC2-F |
5′- gtttagaggagatatcgcgatg-3′ |
This study |
aacC2-R |
5′- ttgtcgacggcctctaac-3′ |
This study |
CTX-M-14-F |
5′- gagagtgcaacggatgatg -3′ |
This study |
CTX-M-14-R |
5′- tgcggctgggtaaaatag -3′ |
This study |
OXA-48-F |
5′- gcttccaccctaatttgatg -3′ |
This study |
OXA-48-R |
5′- cgagcatcagcattttgtc -3′ |
This study |