Table 1. Sample incubation conditions and pyrotag yields.

Sample Name	Avg. Temp. (°C)a	Site	Enrichment	Incubation Location	Ni c	Nf d
UW	81	Water	None	nab	nab	11,233
U77	74	77	None	nab	nab	11,308
U85	83	85	None	nab	nab	14,516
77AS	77	77	Aspen	Sediment	29,188	21,006
77AW	77	77	Aspen	Water	25,528	18,924
77CS	77	77	Corn Stover	Sediment	17,266	12,128
77CW	77	77	Corn Stover	Water	14,527	10,565
85AS	85	85	Aspen	Sediment	7,094	4,842
85AW	85	85	Aspen	Water	12,167	8,259
85CS	85	85	Corn Stover	Sediment	20,416	12,868
85CW	85	85	Corn Stover	Water	32,241	21,349

^aAverage temperature of natural samples or as recorded during incubation (Figure 1).

^bna, not applicable.

^cNumber of pyrotags generated by pyrosequencing.

^dNumber of quality-filtered pyrotags used in analysis.