Figure 2. Studying the interaction of Arl1/GRIP by using GST-GRIP as bait and Arl1-GFP as prey in IBRP assay.

(a) GST-GRIP or Y2177A mutant was immobilized onto beads at 16 µg/µl. The beads were incubated with the cell lysate containing Arl1-GFP (in the presence of 100 µM GMPPNP or GDP) or GFP (as a negative control) and imaged under phase contrast (left column) or fluorescence (right column) setting. In the right column, the fluorescence images were linearly scaled for a fair visual comparison of intensity. Scale bar, 100 µm. (b) Relative IBRP affinity of each interaction. Error bars represent standard deviations. n indicates the number of beads quantified. p indicates the p value of selected pair calculated by t-test.