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. 2013 Mar 29;8(3):e59727. doi: 10.1371/journal.pone.0059727

Figure 4. Studying the interaction of Arl1/GRIP by using GST-Arl1 as bait and GFP-GRIP as prey in IBRP assay.

Figure 4

GST-Arl1 immobilized on beads (13 µg/µl) was loaded with either GMPPNP or GDP. The beads were incubated with cell lysate containing the following GFP-prey: GFP-GRIP, GFP-GRIP Y2177A or GFP (as a negative control). Relative IBRP affinities were culaculated and plotted. Error bars represent standard deviations. n indicates the number of beads quantified. p indicates the p value of selected pair calculated by t-test.