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. 2013 Mar 29;8(3):e60781. doi: 10.1371/journal.pone.0060781

Figure 3. YCP binds to B cells in a saturable and reversible manner.

Figure 3

A and B: Saturable binding kinetics of YCP in B cells. Splenic B cells were incubated with fl-YCP at the indicated concentrations for 1 h at 4°C. The mean fluorescence intensity of each group was examined by flow cytometry. The histogram overlays shown in panel A are representative of triplicates, and the combined results are presented in panel B. C and D: Competitive inhibition of fl-YCP binding to B cells by unlabeled YCP. Cells were incubated with 100 nM of fl-YCP for 1 h in the absence or presence of unlabeled YCP at concentrations from 200 to 3 200 nM, and then harvested for flow cytometric analysis. The histogram overlays shown in panel C are representative of triplicates, and the combined results expressed as a percentage mean fluorescence intensity of fl-YCP with no unlabeled YCP are presented in panel D.