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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Spine J. 2013 Mar;13(3):235–242. doi: 10.1016/j.spinee.2012.01.022

Figure 3.

Figure 3

Biochemical assays showing decreased GAG content of discs in rotating culture conditions, while no significant change in Hypro content was observed. Organ cultured mouse IVDs were harvested at 2, 4, 6, and 8 weeks, and digested with papain solution. The DNA content was measured with Hoechest dye using calf thymus DNA as a standard. The GAG and Hypro contents were measured by dimethylmethylene blue colorimetric assay and dimethylamino-benzaldehyde /chloramine T colorimetric assay, respectively. Values represent mean ±SEM (n=6). *, p<0.05 compared to the static culture condition.