Fig. 4.

Strain is dominant over microtopography in regulating gene expression. RT-PCR of paxillin, vinculin, and integrin-α2 from focal adhesions; caldesmon-1 and calponin3 from actin proteins; and MMP13 from matrix; MMP13 had the highest fold change with post-strain, but the flat-strain and post-strain affected rest of the genes similarly. However, integrin-α2 was slightly decreased with microtopography. Mean ± SE, n = 4. *All vs. flat, ^#FS vs. PS, p < 0.05.