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Basic analysis of atherosclerotic lesions in mice
| Parameter | Tissue | Staining |
|---|---|---|
| Lesion size | Cross-sections/ entire aorta for en face lesion analysis |
HE, (ORO for en face analysis) |
| Neutral lipids | Cross-sections | Oil Red O (ORO) |
| Collagen | Cross-sections | Sirius Red |
| Macrophages | Cross-sections | IHC (CD68 or MOMA-2 antibody) |
| Smooth muscle cells (SMC) |
Cross-sections | IHC (SMC α-actin antibody) |
IHC: Immunohistochemistry.
