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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Methods Mol Biol. 2012;882:531–547. doi: 10.1007/978-1-61779-842-9_30

Table 1a.

Example of how a patient typing is evaluated.

Patient typing: A*02:05, 68:04; B*35:DNXN, 53:01; DRB1*03:01, 13:03; C*04:01; DQB1*02:01, 02:02
Issue noted Web site providing information Action/Resolution
Is information included on all the loci required for matching? If the transplant center would like to search for a 10/10 match, assignments must be included for 5 loci; for a 8/8 match, 4 loci. The typing includes all the required loci for either level of match.
Evaluate resolution of testing:
Allele B*35:DNXN has an allele code		 http://Bioinformatics.nmdp.org DNA Type Lookup Tool Expand to full allele string. Depending on the assignments, request additional typing to increase the resolution. In this case, the assignment includes only alleles that share the sequence of exons 2 and 3: B*35:01, B35:40N, B35:42, B35:57, B35:94.
Evaluate resolution of testing:
C*04:01 allele is at high resolution but this level of resolution required special testing by the HLA laboratory		 http://hla.alleles.org/alleles/index.html G groups Ask the laboratory if it specifically excluded the other alleles that carry the same nucleotide sequence as C*04:01 in the HLA-C exons specifying the peptide binding site. For example, did the laboratory specifically exclude the non- expressed allele C*04:09N? (see Note 46)
Evaluate frequency of alleles:
Allele A*68:04 is uncommon		 http://Bioinformatics.nmdp.org Rare Allele List In family? If yes, acceptable. If no, repeat HLA typing of HLA-A preferably with different reagents/method.
Investigate B/C and DR/DQ haplotypes:
B *35:DNXN with C *04:01
B*53:01 with C*04:01
DRB1*03:01 with DQB1*02:01 or *02:02
DRB1*13:03 with DQB1*02:01 or *02:02		 http://www.haplostats.org/home.do Concordant with family typing? Common or uncommon? In this case, the B-C associations are common. Both DRB1 alleles are frequently observed with DQB1*02:01 but not with DQB1*02:02 so this is unusual. The laboratory should be asked to confirm the DQB1*02:02 assignment if it is not observed in the family.
Investigate multi-locus haplotypes (A/B/C/DRB1/DQB1) http://www.haplostats.org/home.do Concordant with family typing? Common or uncommon?