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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Methods Mol Biol. 2012;882:531–547. doi: 10.1007/978-1-61779-842-9_30

Table 1b.

Examples of how a potential donor typing is evaluated for the patient in Table 1a.

Patient typing: A*02:05, 68:04; B*35:DNXN, 53:01; DRB1*03:01, 13:03; C*04:01
Donor 1 Typing: A2, 68; B35, 53; DRB1* 03:DHJB, 13:BGTY
Issue noted Web site providing information Action/Resolution
Allele codes need expansion http://Bioinformatics.nmdp.org DNA Type Lookup Tool Haplostats DHJB: 03:01/03:06/03:18/03:19/03:22/03:28/03:37
BGTY: 13:03/13:33/13:66
The patient’s DR alleles are included in the donor assignments and, based on the frequency of the alleles in the code, are likely to be matched (i.e., DRB1*13:03 is more common than DRB1*13:33 or DRB1*13:66).
Determine likelihood for allele match:
Serologic typing at HLA-A and B		 Haplostats
HLA Explorer
DNA Dictionary		 Predict higher resolution and likelihood of allele match with patient based on ethnicity. A*02:01 is more frequent than A*02:05; it is unlikely that the donor carries A*6804. B*35:01 is common and may be present in B35 typed individual although other B*35 alleles (B*35:02, B*35:03) are possible; B*53:01 is likely to be present.
Evaluate typing for completeness:
HLA-C not typed		 http://bioinformatics.nmdp.org/index.html, Haplotype table Predict HLA-C alleles that might be present. If B*35:01 and B*53:01 are present, both B alleles are frequently found with C*04:01.
Is resolution sufficient to make a determination of match? The donor is likely an HLA-A mismatch (i.e., not A*68:04). A donor matched at 7/8 or 9/10 may be acceptable to the transplant center. Depending on other donors available on the search report, determine whether this donor should be typed at higher resolution.