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. 2013 Jan 16;83(4):635–646. doi: 10.1038/ki.2012.447

Figure 1.

Figure 1

Adrenocorticotropic hormone (ACTH) gel treatment significantly improves survival and ameliorates systemic organ injury in tumor necrosis factor (TNF)–injured rats. (a) Rats were pretreated subcutaneously with saline or ACTH gel at indicated doses 60 min before intravenous bolus injection of recombinant rat TNF (2 mg/kg wt). Rats were evaluated hourly and survival was recorded. Survival rate was plotted against the time course. The survival probability of rats receiving different treatment was determined by Kaplan–Meier survival analysis. P=0.0040 among the groups by log-rank test. (b–d) Rats were pretreated subcutaneously with saline or ACTH gel (10 IU/kg) 60 min before intravenous bolus injection of recombinant rat TNF (2 mg/kg wt). (b) Mean arterial pressure (MAP) was measured and rats were killed 8 h later. (c) Blood was collected and serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured to assess acute liver injury. (d) Lung was excised and wet to dry lung weight ratio was determined to assess acute lung injury. *P<0.05 versus TNF-alone-treated group (n=5).