Figure 3.

Adrenocorticotropic hormone (ACTH) gel therapy ameliorates histological acute kidney injury in tumor necrosis factor (TNF)–injured rats. Rats were pretreated subcutaneously with saline or ACTH gel (10 IU/kg) 60 min before intravenous bolus injection of recombinant rat TNF (2 mg/kg wt). (a) Rats were killed 8 h later and kidney sections were prepared for hematoxylin and eosin (H&E) staining to evaluate histological injury; original magnification, × 200 (high-power view, x400); necroapoptotic nuclei highlighted by arrowheads. G, glomerulus. Semiquantitative ((b) tubulointerstitial injury score and (d) tubular dilation/sloughing score), as well as computerized ((c) vacuolization area and (e) necroapoptotic nuclei), morphometric analysis was used to estimate kidney injury. *P<0.05 versus other groups (n=5).