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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: J Am Chem Soc. 2013 Feb 19;135(8):2931–2934. doi: 10.1021/ja3122284

Figure 3.

Figure 3

(A) Design of an uppercase “I” and lower case “i” as revealed by atomic force microscopy. (B) First incubation of functionalized tiles with streptavidin, revealing the capital “I” feature. (C) Subsequent incubation with excess biotin selectively removes streptavidin bound to desthiobiotin modifications, revealing the lower case “i.” (D) After washing, newly introduced streptavidin is allowed to bind to free desthiobiotin sites, recovering the capital “I” pattern on the DNA tile. Scale bar = 70 nm.