Table 1.
Primer sequences for RT-PCR analysis
| Gene | Primer | Accession # |
|---|---|---|
| MMP1 | Forward: 5′- GGGAGCAAACACATCTGACCTA-3′ | NM_002421 |
| Reverse: 5′- GCTGGACAGGATTTTGGGAACG-3′ | ||
| MMP3 | Forward: 5′- GTCTCTTTCACTCAGCCAACAC-3′ | NM_002422 |
| Reverse: 5′- CAGCTCGTACCTCATTTCCTCT-3′ | ||
| MMP13 | Forward: 5′- ACTTTATGCTTCCTGATGACGATG-3′ | NM_002427 |
| Reverse: 5′- TGCTGTATTCAAACTGTATGGGTC-3′ | ||
| COL2A1 | Forward: 5′- GAGCAGCAAGAGCAAGGAGAAGA-3′ | NM_001844, NM_033150 |
| Reverse: 5′- CAATGATGGGGAGGCGTGAGGTC-3′ | ||
| Aggrecan | Forward: 5′- TCGTGGTGAAAGGCATCGTGTT-3′ | NM_001135, NM_013227 |
| Reverse: 5′- TCATAGGTCTCGTTGGTGTCTC-3′ | ||
| ADAMTS5 | Forward: 5′- GATGTCTGTGCTCGCCTGTGGT-3′ | NM_007038 |
| Reverse: 5′- CTGTGCAGTAGCGTCCGTTGTT-3′ | ||
| GAPDH | Forward: 5′- ACCACAGTCCATGCCATCAC-3′ | NM_002046 |
| Reverse: 5′- TCCACCACCCTGTTGCTGTA-3′ |