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. Author manuscript; available in PMC: 2013 Mar 31.
Published in final edited form as: J Mol Neurosci. 2010 Mar 17;42(2):183–191. doi: 10.1007/s12031-010-9343-z

Figure 2.

Figure 2

HIF-1α staining is not present in RGC. RGC were previously backlabeled with Fluoro-Gold (a, d, and g). Fluoro-Gold images were converted to gray scale to optimize visualization to determine colocalization with HIF-1α. b No nonspecific staining was observed when sections were incubated with secondary antibody alone. Subtle HIF-1α staining was observed in the inner layers of the normal retina (e) and was increased under conditions of high IOP (h). HIF-1α staining (red) did not colocalize with the RGC marker Fluoro-Gold (white) in the normal retina (f) or in the retina under conditions of high IOP (i). Insets are higher magnification views of the RGC indicated by arrows