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. 2013 Mar 14;2013:364519. doi: 10.1155/2013/364519

Table 1.

Differentially expressed proteins were listed alphabetically after 2D-DIGE and MALDI-TOF mass spectrometry analysis in H9C2 cells in response to H2O2 treated and pretreated with quercetin. The average ratios of these 44 spots are differentially expressed between untreated (control), H2O2-treated, and quercetin-pretreated followed by treatment with H2O2, calculated from triplicate gels. Boldface numbers represent proteins for which the changes between the H2O2 treatment and the control are significantly greater than changes between quercetin pretreatment followed by H2O2 treatment and control.

Spot no. Swissprot no. Protein name Pred. MW Pred. PI Cov. % MASCOT score No. of peptides
match/
supplied		 H2O2/Ctrl Quercetin + H2O2/Ctrl Peptide sequence Function
1420 P63324 40S ribosomal protein S12 14858 6.82 42% 55/51 6/30 −1.25 −2.77 QAHLCVLASNCDEPMYVK,
TALIHDGLAR		 Protein synthesis
691 Q9JLJ3 4-Trimethylaminobutyraldehyde dehydrogenase 54530 6.57 44% 109/56 16/66 1.68 1.9 AFEPATGR,
AGAPNGLFNVVQGGAATGQFLCQHR		 Protein synthesis
473 P63039 60 kDa heat shock protein (mitochondrial) 61088 5.91 20% 61/56 8/30 1.37 1.54 AAVEEGIVLGGGCALLR,
ISSVQSIVPALEIANAHR		 Chaperon
1010 P60711 Actin (cytoplasmic) 42052 5.29 23% 54/51 6/37 −1.51 −1.27 GYSFTTTAER,
SYELPDGQVITIGNER		 Cytoskeleton
704 P11884 Aldehyde dehydrogenase, mitochondria 56966 6.63 41% 143/56 16/54 1.12 1.56 RVTLELGGK,
SGQQEGAK		 Redox regulation
990 P54921 Alpha-soluble NSF attachment protein 33627 5.3 50% 120/56 13/39 9.85 7.69 QAEAMALLAEAER,
IEEACEIYAR		 Transport
1002 P55260 Annexin A4 36168 5.31 39% 145/51 13/28 −1.92 −2.35 GDTSGDYR,
WGTDEVK		 Transport/Ca2+
396 P48037 Annexin A6 76106 5.39 52% 253/56 36/74 −1.35 −1.73 YELTGKFER,
AINEAYKEDYHK		 Transport/Ca2+
1116 P35426 Cell division protein kinase 4 34006 6.09 54% 198/56 15/32 3.5 3.22 VTLVFEHIDQDLR,
VPNGGAAGGGLPVSTVR		 Cell cycle
1319 P47875 Cysteine and glycine-rich protein 1 21455 8.9 51% 58/56 6/44 −7.38 −11.9 NLDSTTVAVHGEEIYCK,
GLESTTLADKDGEIYCK		 Cytoskeleton regulation
1323 P47875 Cysteine and glycine-rich protein 1 21455 8.9 54% 91/56 7/40 13.77 12.77 TVYFAEEVQCEGNSFHK,
HEEAPGHRPTTNPNASK		 Cytoskeleton regulation
416 Q5XI50 E3 ubiquitin-protein ligase MARCH7 76932 7.64 16% 53/51 9/44 −2.86 −3.98 MVSGNRGTSLNDSYHSR,
CTGSLQYVHQECMK		 Protein degradation
709 P62630 Elongation factor 1-alpha 1 50424 9.1 29% 87/56 11/35 −2.01 −1.1 EHALLAYTLGVK,
STTTGHLIYK		 Protein synthesis
1244 O08719 Ena/VASP-like protein 42183 8.74 20% 59/51 6/43 2.27 1.63 WVPIKPGQQGFSR,
VKPAGSVNDVGLDALDLDRMK		 Cytoskeleton regulation
305 Q99PF5 Far upstream element-binding protein 2 74466 6.38 41% 169/56 20/43 −2.09 −2.25 ERDQGGFGDR.
IGQQPQQPGAPPQQDYTK		 Gene expression
886 P97590 Galectin-7 15333 6.43 29% 52/51 6/43 −1.41 −1.58 MPSSNVRSVEVGGDVQLHSVK,
MSATHHK		 Apoptosis
1251 Q9Z1B2 Glutathione S-transferase Mu 5 27067 6.33 50% 76/56 15/54 −4.85 −9.44 ITQSNAILR,
VDIMENQIMDFR		 Redox regulation
1255 P42930 Heat shock protein beta-1 22936 6.12 56% 147/56 12/65 −3.9 −4.49 KYTLPPGVDPTLVSSSLSPEGTLTVEA,
VPFSLLR		 Chaperon
954 Q6RUG5 Islet cell autoantigen 1-like protein 49298 5.23 31% 54/51 9/53 −1.64 −1.6 MDSFEHLRPEDSQSVVSRMQK,
DASQELDPDTFK		 Unknown
1240 O35760 Isopentenyl-diphosphate Delta-isomerase 1 26721 5.57 38% 61/56 6/58 −1.8 −1.31 MPEINASNLDEK,
AELGIPLEEVDLNEMNYLTR		 Lipid synthesis
1237 Q63279 Keratin, type I cytoskeletal 19 44609 5.21 25% 51/51 10/73 4.02 3.7 QGPGPFRDYSQYFK,
MSVEADINGLRR		 Cytoskeleton
1219 Q6QLM7 Kinesin heavy chain isoform 5A 117642 5.56 15% 54/51 12/43 −2 −2.3 SLTEYMQTVELKK,
MAETNNECSIKVLCR		 Transport
418 P56536 Kinesin heavy chain isoform 5C (Fragment) 27376 5.87 25% 63/51 6/40 −2.7 −3.79 FVSSPEEVMDVIDEGK,
NRHVAVTNMNEHSSR		 Transport
349 P48679 Lamin-A 74564 6.54 48% 221/56 32/68 −2.04 −2.28 LQDEMLRR,
LESSESR		 Cytoskeleton
1263 Q6AYP2 Microfibrillar-associated protein 3-like 45804 4.9 13% 55/51 7/30 1 2.22 DEVYTIPNSLKR,
VTQFKTMEFAR		 Sperm development
1355 P13832 Myosin regulatory light chain RLC-A 19940 4.67 62% 102/56 13/52 2.18 1.82 DGFIDKEDLHDMLASMGK,
GNFNYIEFTR		 Muscle contraction
1357 Q64122 Myosin regulatory light polypeptide 9 19765 4.8 42% 68/51 10/48 2.12 1.5 EAFNMIDQNR,
KGNFNYVEFTR		 Muscle contraction
1276 Q63716 Peroxiredoxin-1 22323 8.27 41% 77/56 7/44 −1.84 −2.56 ADEGISFR,
MSSGNAKIGHPAPSFK		 Redox regulation
1213 P97562 Peroxisomal acyl-coenzyme A oxidase 2 77548 7.64 17% 53/51 10/41 2.19 1.96 HGMHAFIVPIR,
LAWSLGWSEDGPER		 lipid metabolism
1198 P25113 Phosphoglycerate mutase 1 28928 6.67 40% 72/51 8/28 −1.66 −5.8 YADLTEDQLPSCESLKDTIAR,
VLIAAHGNSLR		 Glycolysis
1202 P25113 Phosphoglycerate mutase 1 28928 6.67 60% 169/51 22/59 1.41 1.56 HGESAWNLENR,
FSGWYDADLSPAGHEEAK		 Glycolysis
1405 P62963 Profilin-1 15119 8.46 75% 94/56 11/44 −3.01 1.12 EGVHGGLINK,
EGVHGGLINKK		 Cytoskeleton regulation
1120 P18420 Proteasome subunit alpha type-1 29784 6.15 44% 115/56 12/28 −1.37 −1.53 NQYDNDVTVWSPQGR
QECLDSR		 Protein degradation
1242 P40112 Proteasome subunit beta type-3 23235 6.15 43% 60/51 9/50 −3.09 −1.49 LNLYELKEGR,
NCVAIAADRR		 Protein degradation
1300 P34067 Proteasome subunit beta type-4 29349 6.45 34% 70/56 12/62 6.23 6.45 FDCGVVIAADMLGSYGSLAR,
VNDSTMLGASGDYADFQYLK		 Protein degradation
1321 P28075 Proteasome subunit beta type-5 28738 6.52 35% 75/56 8/48 15.04 13.13 GMGLSMGTMICGWDKR,
RGPGLYYVDSEGNR		 Protein degradation
522 P11598 Protein disulfide-isomerase A3 57044 5.88 26% 77/56 11/38 1.31 1.69 GFPTIYFSPANK,
IFRDGEEAGAYDGPR		 Redox regulation
1301 Q6IML7 Rab and DnaJ domain-containing protein 31329 8.72 26% 54/51 6/30 −3.01 −2.61 EPLKSLR,
CIDESEGRLWAESR		 Signal transduction
781 P29315 Ribonuclease inhibitor 51653 4.67 57% 174/56 18/64 −1.56 −1.42 LSLQNCSLTEAGCGVLPDVLR,
LQLEYCNLTATSCEPLASVLR		 Gene expression
952 P62138 Serine/threonine-protein phosphatase PP1-alpha catalytic subunit 38229 5.94 53% 163/56 16/49 −1.44 −1.52 TFTDCFNCLPIAAIVDEK,
IYGFYDECK		 Signal transduction
397 P48721 Stress-70 protein (mitochondrial) 74097 5.97 33% 103/56 21/79 −2.12 −4.1 VCQGER,
DNMALQR		 Chaperon
437 O35814 Stress-induced-phosphoprotein 1 63158 6.4 20% 88/51 12/62 1.26 1.56 AAALEFLNR,
TLLSDPTYR		 Transport
1300 P83941 Transcription elongation factor B polypeptide 1 12636 4.74 45% 56/56 5/62 6.23 6.45 AMLSGPGQFAENETNEVNFR,
EIPSHVLSKVCMYFTYK		 Gene expression
1208 Q91Y78 Ubiquitin carboxyl-terminal hydrolase isozyme L3 26278 5.01 63% 124/56 13/43 −1.64 −1.62 HLENYDAIR,
VDLHFIALVHVDGHLYELDGR		 Protein degradation