Table 1.

Primers used in the amplification of the gene regions

Gene	Primer sequences	Amplicon size
HLA-DRB1 exon 2
Forward primer	5’-cagcacgtttcctgtggcag-3’	261 bp
Reverse primer	5’-ctgtgaagctctccacaaccc -3’
TNF-α promoter (-308)
Forward primer	5’-gaaggaaacagaccacagacc -3’	187 bp
Reverse primer	5’- ggggacacacaagcatca -3’
ITPKC intron 1 [rs28493229]
Forward primer	5’-ctggcactggtggtttccaaat-3’	500 bp
Reverse primer	5’-aagaggttcccggagatgaaattg-3’