Figure 3.

Reprogrammed liver-IPCs are glucose responsive. A. IPGTT after subtotal Px. After recovery from surgery around 22-24 days post-Px, mice were injected with glucose (2 mg/g body weight i.p.) and the blood glucose levels were recorded at indicated times. B. Expression of glucagon and insulin genes in the liver. Expression of glucagon and insulin mRNAs was assayed in the livers of normal mice (L1), or Stz/diabetic mice treated with GFP (L2) or Pdx1/Ngn3 (L3) by RT-PCR. Pancreatic tissue (P) serves as positive control. C. & D. Immunohistochemical analysis of liver sections. Liver sections harvested from mice at day 24 post-AAV-treatment (as indicated) were immunostained with anti-insulin (C) or anti-insulin and glucagon (D) antibodies. Islet in the mouse pancreatic tissue serves as positive controls. Either insulin-positive (green) or glucagon-positive (red) or both-positive liver cells are indicated by arrows. Original magnification is 40x.