a) Surface expression of α4β7 and CCR9 on live lin− CD11c+B220+ DCs from lymphoid and non-lymphoid tissues from 2–3 week old C57Bl/6 mice; lin indicates CD3, CD19, and NK1.1. Data are representative of seven independent experiments. b) Surface expression of α4β7 and CCR9 on live lin−CD11c+B220+ DCs from lymphoid and non-lymphoid tissues taken from Flt3L-treated mice. Data are representative of at least three independent experiments. c) Three million purified pre-μDCs, sorted from peripheral lymphoid tissues from Flt3L-treated B6.CD45.2 mice, were transferred intravenously into B6.CD45.1 recipients. Tissues were harvested on day 3 after transfer and donor-derived cells were quantified. Data are presented as the percentage of total CD11c+ host cells. Each dot represents 1 individual animal, n=3 from two independent experiments. p<0.01 for LP vs. all other tissues by Student’s t-test.