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Agrose gel electrophoresis of digested low molecular polypeptide-7 amplicons. Purified genomic DNA from each patient was subjected to polymerase chain reaction amplification using specific primers bracketing the low molecular polypeptide-7 gene followed by BsmI digestion and the products were resolved on 2% agarose gel. Lanes 2, 3 and 6 represent heterozygous C/A; Lanes 1 and 5 show C/C genotype; Lane 4 shows A/A genotype; M indicated molecular weight marker.
