Fig. 1.

ESI-MS molecular ion profiles of GSLs from non-CSCs and CSCs. Full spectrums for non-CSCs and CSCs were separated into two groups, m/z 980–1,530 and m/z 1,530–2,080 as indicated. (A–D) Annotations of GSLs on the spectrum were assigned based on m/z values typical for ceramide moiety-associated fatty acyl heterogeneity. We deduced that GSLs with the same glycan moiety but different fatty acyl components are the same GSL. NL represents a measure of absolute spectral counts for the most abundant peak (100% on the y-axis) in respective segments.