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. 2013 Mar 11;110(13):4968–4973. doi: 10.1073/pnas.1302825110

Fig. 5.

Fig. 5.

Knockdown of ST8SIA1, B4GALNT1, or ST3GAL5 reduces mammosphere formation in MCF-7 cells. (A) Real-time RT-PCR illustrates a reduction in mRNA levels after transfection of shRNA against ST8SIA1 or B4GALNT1. Relative expressions for target genes are shown on the y-axis. (B) Expression of GD2 or GD3 decreased after knockdown of ST8SIA1 and B4GALNT1. GD2 or GD3 expression (y-axis) and forward scatter (FSC) (x-axis) are shown. Percentages represent GSL-positive cells plots. (C) Mammosphere formation of mock control, vector control, ST8SIA1-KD, or B4GALNT1-KD cells. Microscope magnification was ×200. Data represent the mean of three independent experiments. ns, not significant; **P ≤ 0.005. (Scale bar, 0.1 mm.)