Fig. 1.

BamA exists in two conformations. (A) Cell pellets from wild-type, bamDR197L, and ΔbamE strains were treated with proteinase K where indicated. The protease was inactivated by treatment with PMSF, and samples were analyzed by SDS/PAGE and immunoblot using anti-BamA and anti-MBP antibodies. MBP serves as a loading control to ensure that protease does not enter the periplasm. (B) Wild-type, bamDR197L, and ΔbamE cells were processed by gentle lysis and treated at the indicated temperatures. Samples were analyzed by SDS/PAGE and immunoblot using anti-BamA and anti-BamC antibodies.