Fig. 4.

BamA L6 and BamDE communicate via POTRA5. (A) A His-tagged variant of BamD was expressed in bamA⁺ or bamA E373A strains to enable copurification of the Bam complex. Extracts of each strain were purified using a Ni-NTA affinity resin and eluted with imidazole. Input and eluted fractions were analyzed by SDS/PAGE and immunoblot with antibodies that recognize BamA, BamC, or BamD. (B) Strains carrying bamAE373A, bamAE373A+Q693P, bamAE373A+C700S, or bamAE373A+C690S+C700S mutations were streaked onto LB agar plates supplemented with 0.5% SDS and 1.0 mM EDTA. The image was captured after overnight growth at 37 °C.