Fig. 6.

PGJ₂ signal inhibits cytokine expression in endothelial cells and neutrophil migration, and DP agonism enhances endothelial barrier formation. (A) mRNA expression of TNF-α in mouse lung endothelial cells, shown as the ratio of GAPDH (n = 5 each). (B and C) TER; typical responses are shown in B, and data are summarized in C (n = 4–6). (D) Transmembrane migration assay performed using isolated neutrophils. LTB₄ was added to the lower chamber and test agents were added to both the lower and upper chambers. Data represent the number of migrating cells to lower chambers in one field (20×; n = 5 each). *^,†P < 0.05 compared with nontreated or LPS-treated cells.