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. 2013 Mar 14;110(13):5223–5228. doi: 10.1073/pnas.1214530110

Fig. 4.

Fig. 4.

Q141K does not disrupt interdomain interactions or dimerization. Western blots of total (A) and dimer (B) expression of Q141K ABCG2 with secondary mutations at K473 demonstrating that K473W cannot rescue Q141K expression. Summary data of K473 mutation on total (C; n = 4–6) Q141K expression. (D) Expression of total or dimer Q141K as a fraction of wild type (total or dimer); a greater proportion of total Q141K exists as a dimer than does wild type (n = 7). (E) Superimposition of ABCG2 NBD model (green, with Q141 as orange sticks and F142 as red sticks) and the model with Q141K substitution (blue, with K141 and F142 as cyan sticks). The Sav1866 crystal (PDB ID: 2HYD) was also superimposed, and one of its intracellular loops (dark green) was shown to indicate possible NBD–TMD interface mediated by F142. All means are ± SEM; *P < 0.05; **P < 0.01.