Table 1.

Comparison of production of human milk recombinant proteins (RPs) in transgenic animals (TAs) using various variants of the gene construct

RP/construct	Regulatory elements	TA/method of production	Maximum level of RP production, mg/ml	Reference
Lactoferrin
Native gene	Bacmid	Cow/NT	3.4	[16]
Native gene	WAP-gene (21 kb) (mouse)	Mouse/MI	30	[21]
–“–	β-casein promoter (goat) + insulator	Mouse/MI	160	[22]
–“–	β-casein promoter (goat) + insulator	Goat/MI	10.8	[22]
–“–	αS1-casein promoter (cow)	Cow/MI	3	[23]
cDNA	β-casein promoter (goat) + insulator	Goat/MI	0.7	[24]
cDNA	αS1-casein promoter (cow)	Mouse/MI	0.036	[25]
–“–	β-casein promoter (goat) + insulator	Mouse/MI	4	[26]
Lysozyme
cDNA	β-casein promoter (goat) + insulator	Cow/NT	0.026	[17]
–“–	β-casein promoter (goat) + insulator	Pig/NT	0.00032	[27]
–“–	α-S1- casein (cow)	Goat/MI	0.27	[28]
–“–	α-S1- casein (cow)	Mouse/MI	0.00071	[29]
α-Lactalbumin
Native gene	Bacmid	Cow/NT	1.55	[30]