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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Exp Mol Pathol. 2012 Apr 16;93(1):40–49. doi: 10.1016/j.yexmp.2012.04.002

Fig. 2.

Fig. 2

The effect of rapamycin treatment on oval cell abundance in rats placed on the modified CDE protocol. (A) Western immunoblotting for phospho-S6Ser235/236 and total S6 was performed on total liver homogenates from rats placed on the CDE protocol, CDE plus vehicle (CDE-V), or CDE in combination with rapamycin treatment (CDE-Rapa). (B) Liver cryosections underwent immunofluorescent staining for an oval cell marker BD.2 and were counterstained with DAPI. Images were acquired at 40×. Scale bar: 50 μm. The graph shows the percent area of BD.2-positive cells in ten 20× fields. Results are shown as individual data points and the mean. ◇, CDE; △, CDE-V; ○, CDE–Rapa. *; p=0.003.